Warfarin Sodium clathrate is an anticoagulant that inhibits the synthesis of vitamin K-dependent coagulation factors. As per literature survey, there is no UV AUC method has been reported for estimation of warfarin sodium clathrate in bulk form and in pharmaceutical formulation. Hence an attempt has been made to develop and validate a simple, economic, rapid and accurate method. The linearity for this method was found to be in the range of 10-30 μg/ml. The calibration curve was drawn by plotting graph between AUC and concentration. Coefficient of correlation was found to be 0.9999. The regression of the curve was Y = 0.0101x + 0.009. The lower limit of detection and the limit of quantification were found to be 0.1146 μg/ml and 0.5049 μg/ml respectively. The percentage recovery was found to be 97.09±0.2411.
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